The State Museum, State Library and State Archives are temporarily Closed.

Peptide Sequences from the First Castoroides ohioensis Skull and the Utility of Old Museum Collections for Palaeoproteomics

TitlePeptide Sequences from the First Castoroides ohioensis Skull and the Utility of Old Museum Collections for Palaeoproteomics
Publication TypeJournal Article
Year of Publication2016
AuthorsCleland, TP, Schroeter, ER, Feranec, RS, Vashishth, D
JournalProceedings of the Royal Society B: Biological Sciences
Volume283
Issue1832
Pagination20160593
Date PublishedMar-06-2017
ISSN0962-8452
Abstract

Vertebrate fossils have been collected for hundreds of years and are stored in museum collections around the world. These remains provide a readily available resource to search for preserved proteins; however, the vast majority of palaeoproteomic studies have focused on relatively recently collected bones with a well-known handling history. Here, we characterize proteins from the nasal turbinates of the first Castoroides ohioensis skull ever discovered. Collected in 1845, this is the oldest museum-curated specimen characterized using palaeoproteomic tools. Our mass spectrometry analysis detected many collagen I peptides, a peptide from haemoglobin beta, and in vivo and diagenetic post-translational modifications. Additionally, the identified collagen I sequences provide enough resolution to place C. ohioensis within Rodentia. This study illustrates the utility of archived museum specimens for both the recovery of preserved proteins and phylogenetic analyses.

URLhttp://rspb.royalsocietypublishing.org/lookup/doi/10.1098/rspb.2016.0593
DOI10.1098/rspb.2016.0593
Short TitleProc. R. Soc. B