Peptide Sequences from the First Castoroides ohioensis Skull and the Utility of Old Museum Collections for Palaeoproteomics
|Title||Peptide Sequences from the First Castoroides ohioensis Skull and the Utility of Old Museum Collections for Palaeoproteomics|
|Publication Type||Journal Article|
|Year of Publication||2016|
|Authors||Cleland, TP, Schroeter, ER, Feranec, RS, Vashishth, D|
|Journal||Proceedings of the Royal Society B: Biological Sciences|
Vertebrate fossils have been collected for hundreds of years and are stored in museum collections around the world. These remains provide a readily available resource to search for preserved proteins; however, the vast majority of palaeoproteomic studies have focused on relatively recently collected bones with a well-known handling history. Here, we characterize proteins from the nasal turbinates of the first Castoroides ohioensis skull ever discovered. Collected in 1845, this is the oldest museum-curated specimen characterized using palaeoproteomic tools. Our mass spectrometry analysis detected many collagen I peptides, a peptide from haemoglobin beta, and in vivo and diagenetic post-translational modifications. Additionally, the identified collagen I sequences provide enough resolution to place C. ohioensis within Rodentia. This study illustrates the utility of archived museum specimens for both the recovery of preserved proteins and phylogenetic analyses.
|Short Title||Proc. R. Soc. B|