02169nas a2200193   4500008004100000245006100041210006100102300001200163490000700175520162800182653001201810100001401822700002001836700001801856700001801874700001601892700001901908856004801927       2015                        eng d00aParasitoid Venom Induces Metabolic Cascades in Fly Hosts0 aParasitoid Venom Induces Metabolic Cascades in Fly Hosts  a350-3660 v113 a<p>Parasitoid wasps inject insect hosts with a cocktail of venoms to manipulate the physiology, development, and immunity of the hosts and to promote development of the parasitoid offspring. The jewel wasp Nasonia vitripennis is a model parasitoid with at least 79 venom proteins. We conducted a high-throughput analysis of Nasonia venom effects on temporal changes of 249 metabolites in pupae of the flesh fly host (<em>Sarcophaga bullata</em>), over a 5-day time course. Our results show that venom does not simply arrest the metabolism of the fly host. Rather, it targets specific metabolic processes while keeping hosts alive for at least 5&nbsp;days post venom injection by the wasp. We found that venom: (a) activates the sorbitol biosynthetic pathway while maintaining stable glucose levels, (b) causes a shift in intermediary metabolism by switching to anaerobic metabolism and blocking the tricarboxylic acid cycle, (c) arrests chitin biosynthesis that likely reflects developmental arrest of adult fly structures, (d) elevates the majority of free amino acids, and (e) may be increasing phospholipid degradation. Despite sharing some metabolic effects with cold treatment, diapause, and hypoxia, the venom response is distinct from these conditions. Because Nasonia venom dramatically increases sorbitol levels without changing glucose levels, it could be a useful model for studying the regulation of the sorbitol pathway, which is relevant to diabetes research. Our findings generally support the view that parasitoid venoms are a rich source of bioactive molecules with potential biomedical applications.</p>
10abiology1 aMrinalini1 aSiebert, A., L.1 aWright, J., J1 aMartinson, E.1 aWheeler, D.1 aWerren, J., H.  uhttp://dx.doi.org/10.1007/s11306-014-0697-z