09429nas a2200253 4500008004100000245010500041210006900146300001200215490000700227520865200234653002208886653002208908653001508930653003208945653002308977653002509000653001809025100001909043700001909062700001909081700001809100700001709118856004009135 2001 eng d00aCharacterization of Intracytoplasmic Prokaryote Infections in Dreissena sp. (Bivalvia: Dreissenidae)0 aCharacterization of Intracytoplasmic Prokaryote Infections in Dr a203-2160 v443 a

This study characterizes intracytoplasmic infections with prokaryote microorganisms in Dreissena sp. (near Dreissena polymorpha) from northeastern Greece and represents the first report of such infections in freshwater bivalves. Light microscope observations of stained tissues revealed basophilic, cytoplasmic inclusion bodies in 87.5% (28/32) of the mussels sectioned. Inclusions in epithelial cells and connective tissues were noted, respectively, in 34.4 and 71.9% of the sample, with 5 mussels (15.6%) having both tissue types infected. Epithelial cell infections were observed in histological sections only in digestive gland tubules and ducts; within tubules, inclusions were present more often in secretory than digestive cells. Connective tissue infections, however, were systemic; among the 32 mussels sectioned, inclusions were found in the gills (65.6%), foot (12.5%), mantle (9.4%), labial palps (6.3%), digestive gland (6.3%), stomach (6.3%), and gonads (3.1%). Cytoplasmic inclusions (maximum dimension, 138 microm) were prominent enough in the gills to be visible in 17.0% of the 247 mussels dissected. Ultrastructurally, prokaryote cells in gill connective tissues were clearly characteristic of Chlamydiales-like organisms, with each intracytoplasmic inclusion containing a loosely packed mixture of elementary, reticulate, intermediate bodies, and blebs. Prokaryote colonies in digestive gland epithelial cells exclusively contained 1 of 4 morphological cell types and were considered Rickettsiales-like. Hexagonal, virus-like particles were present in the cytoplasm of the largest of these Rickettsiales-like prokaryotes. Although host stress was evident from localized cell necrosis and dense hemocyte infiltration, overall infection was fairly benign, with no major, adverse impact on body condition evident among sectioned or dissected mussels. A possible negative effect was partial constriction of gill water tubes, but at the infection intensity observed (typical range 1 to 7 inclusion bodies per section), significant interference with respiration and other metabolic functions of the gills was highly unlikely.

10aChlamydiales-like10aConnective tissue10aEpithelium10aIntracytoplasmic prokaryote10aRickettsiales-like10aVirus-like particles10aZebra mussels1 aMolloy, D., P.1 aGiamberini, L.1 aMorado, J., F.1 aFokin, S., I.1 aLaruelle, F. uhttp://dx.doi.org/10.3354/dao044203