We employ social network analysis of collar decoration on Iroquoian vessels to conduct a multiscalar analysis of signaling practices among ancestral Huron-Wendat communities on the north shore of Lake Ontario. Our analysis focuses on the microscale of the West Duffins Creek community relocation sequence as well as the mesoscale, incorporating several populations to the west. The data demonstrate that network ties were stronger among populations in adjacent drainages as opposed to within drainage-specific sequences, providing evidence for west-to-east population movement, especially as conflict between Wendat and Haudenosaunee populations escalated in the sixteenth century. These results suggest that although coalescence may have initially involved the incorporation of peoples from microscale (local) networks, populations originating among wider mesoscale (subregional) networks contributed to later coalescent communities. These findings challenge previous models of village relocation and settlement aggregation that oversimplified these processes.