Faunal assemblages from the pre-Contact period sites Frisby-Butler and Hornblower II on Martha s Vineyard, Massachusetts, USA, remain unstudied since excavation during the 1980s. This project establishes radiocarbon (14C) dates from faunal remains and evaluates occupation and abandonment at each site. 14C measurements were collected from 17 specimens and 13 dates from previous analyses were re-examined. Dates were identified from the archaeological time periods Transitional Archaic (2700–3700 BP), Early Woodland (2000–2700 BP), and Late Woodland (450–1200 BP) at Frisby-Butler. Occupation likely represented seasonal visitations during autumn and winter to hunt based on white-tailed deer (Odocoileus virginianus) demographic profiles. A combined dataset of new and re-calibrated 14C measurements from Hornblower II date to the Late Archaic (3700–6000 BP), Early Woodland, Middle Woodland (1200–2000 BP), and Late Woodland periods. Settlement was focused on gathering warm-weather foods like demersal fish and lakebirds. Together, the sites demonstrate periodic seasonal use of the southwest coast of the island throughout the Late Holocene and fit within an established regional pattern in southern New England.