Recent establishment of invasive Alosa pseudoharengus (Alewife) in Lake Champlain and in the lake’s watershed has been cause for concern. Based on three larval specimens newly discovered in the New York State Museum collections, we document Alewife in Lake Champlain in 1929. Therefore, there has been at least one early unsuccessful introduction of Alewife into Lake Champlain and there may have been others that escaped notice. Changing lake conditions may have allowed Alewife to recently become established.