Tremadocian onlap is recorded by the Tribes Hill Formation. The formation is a lower Lower Ordovician (upper conodont Fauna B Interval(?)- Rossodus manitouensis Zone) depositional sequence that unconformably overlies the Upper Cambrian Little Falls Formation. Depositional environments and stratigraphy indicate that the Tribes Hill was deposited on a wave-, not tide-, dominated shelf and that a uniform, ’layer-cake’ stratigraphy is present. The deepening-shoaling sequence of the Tribes Hill includes the: 1) Sprakers Member (new; peritidal carbonate and overlying tempestite limestone and shale); 2) Van Wie Member (new; subtidal shale and limestone); 3) Wolf Hollow Member (revised; massive carbonates with thrombolitic cap); and 4) Canyon Road Member (new; glauconitic limestone and overlying evaporitic dolostone). The shoaling half-cycle of the Tribes Hill is older than a shoaling event in western Newfoundland, and suggests epeirogenic factors in earliest Ordovician sea-level change in east Laurentia. Conodont and trilobite biofacies track lithofacies, and Rossodus manitouensis Zone conodonts and Bellefontia Biofacies trilobites appear in the distal, middle Tribes Hill Formation. Twenty-four conodont species are illustrated. Ansella? protoserrata new species, lapetognathus sprakersi new species, Leukorhinion ambonodes new genus and species, and Laurentoscandodus new genus are described.